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nonimmune rabbit serum catalog no. 10510  (Thermo Fisher)


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    Thermo Fisher nonimmune rabbit serum catalog no. 10510
    Nonimmune Rabbit Serum Catalog No. 10510, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonimmune+rabbit+serum/nonimmune+rabbit+serum+catalog+no++10510/pmc11843583-331-0-9
    Average 90 stars, based on 1 article reviews
    nonimmune rabbit serum catalog no. 10510 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Generated:

    Article Title: Dual Roles for Prox1 in the Regulation of the Chicken β B1-Crystallin Promoter
    Article Snippet: .. Lenses and livers were removed from 14-day-old chicken embryos and sheared, and formaldehyde cross-linked chromatin (average size, 600 bp) was generated by sonication, as described in Yang et al. 39 Aliquots of chromatin prepared from 10 lenses or 1 to 2 mg liver were incubated with 1 μ L well-characterized rabbit polyclonal antibody prepared against the homeo- and Prospero domains of Prox1 20 or nonimmune rabbit serum (catalog no. 31884; Pierce, Rockford, IL). .. Antibody–chromatin complexes were captured by the addition of 100 μ L protein G–agarose beads preblocked with salmon sperm DNA (Upstate/Millipore, Billerica, MA).

    Article Title: Dual Roles for Prox1 in the Regulation of the Chicken β B1-Crystallin Promoter
    Article Snippet: .. Oligonucleotides Used for Electrophoretic Mobility Shift Analysis (Sense Strands Only Are Shown) Chromatin Immunoprecipitation Analysis Lenses and livers were removed from 14-day-old chicken embryos and sheared, and formaldehyde cross-linked chromatin (average size, 600 bp) was generated by sonication, as described in Yang et al. 39 Aliquots of chromatin prepared from 10 lenses or 1 to 2 mg liver were incubated with 1 μ L well-characterized rabbit polyclonal antibody prepared against the homeo- and Prospero domains of Prox1 20 or nonimmune rabbit serum (catalog no. 31884; Pierce, Rockford, IL). .. Antibody–chromatin complexes were captured by the addition of 100 μ L protein G–agarose beads preblocked with salmon sperm DNA (Upstate/Millipore, Billerica, MA).

    Sonication:

    Article Title: Dual Roles for Prox1 in the Regulation of the Chicken β B1-Crystallin Promoter
    Article Snippet: .. Lenses and livers were removed from 14-day-old chicken embryos and sheared, and formaldehyde cross-linked chromatin (average size, 600 bp) was generated by sonication, as described in Yang et al. 39 Aliquots of chromatin prepared from 10 lenses or 1 to 2 mg liver were incubated with 1 μ L well-characterized rabbit polyclonal antibody prepared against the homeo- and Prospero domains of Prox1 20 or nonimmune rabbit serum (catalog no. 31884; Pierce, Rockford, IL). .. Antibody–chromatin complexes were captured by the addition of 100 μ L protein G–agarose beads preblocked with salmon sperm DNA (Upstate/Millipore, Billerica, MA).

    Article Title: Dual Roles for Prox1 in the Regulation of the Chicken β B1-Crystallin Promoter
    Article Snippet: .. Oligonucleotides Used for Electrophoretic Mobility Shift Analysis (Sense Strands Only Are Shown) Chromatin Immunoprecipitation Analysis Lenses and livers were removed from 14-day-old chicken embryos and sheared, and formaldehyde cross-linked chromatin (average size, 600 bp) was generated by sonication, as described in Yang et al. 39 Aliquots of chromatin prepared from 10 lenses or 1 to 2 mg liver were incubated with 1 μ L well-characterized rabbit polyclonal antibody prepared against the homeo- and Prospero domains of Prox1 20 or nonimmune rabbit serum (catalog no. 31884; Pierce, Rockford, IL). .. Antibody–chromatin complexes were captured by the addition of 100 μ L protein G–agarose beads preblocked with salmon sperm DNA (Upstate/Millipore, Billerica, MA).

    Incubation:

    Article Title: Dual Roles for Prox1 in the Regulation of the Chicken β B1-Crystallin Promoter
    Article Snippet: .. Lenses and livers were removed from 14-day-old chicken embryos and sheared, and formaldehyde cross-linked chromatin (average size, 600 bp) was generated by sonication, as described in Yang et al. 39 Aliquots of chromatin prepared from 10 lenses or 1 to 2 mg liver were incubated with 1 μ L well-characterized rabbit polyclonal antibody prepared against the homeo- and Prospero domains of Prox1 20 or nonimmune rabbit serum (catalog no. 31884; Pierce, Rockford, IL). .. Antibody–chromatin complexes were captured by the addition of 100 μ L protein G–agarose beads preblocked with salmon sperm DNA (Upstate/Millipore, Billerica, MA).

    Article Title: Influenza A virus exploits the motility of membrane cytoskeletal actomyosin filaments for its genome packaging in the host cell
    Article Snippet: .. After labelling with the primary antibody, the cells were washed with fresh PBS three times for 5 min each and then incubated with 10 nm gold-conjugated goat anti-rabbit IgG (British BioCell International, UK) for 2 h. As a control for immunolabelling, nonimmune rabbit serum (Invitrogen MA USA) was used instead of primary antibody. ..

    Article Title: Dual Roles for Prox1 in the Regulation of the Chicken β B1-Crystallin Promoter
    Article Snippet: .. Oligonucleotides Used for Electrophoretic Mobility Shift Analysis (Sense Strands Only Are Shown) Chromatin Immunoprecipitation Analysis Lenses and livers were removed from 14-day-old chicken embryos and sheared, and formaldehyde cross-linked chromatin (average size, 600 bp) was generated by sonication, as described in Yang et al. 39 Aliquots of chromatin prepared from 10 lenses or 1 to 2 mg liver were incubated with 1 μ L well-characterized rabbit polyclonal antibody prepared against the homeo- and Prospero domains of Prox1 20 or nonimmune rabbit serum (catalog no. 31884; Pierce, Rockford, IL). .. Antibody–chromatin complexes were captured by the addition of 100 μ L protein G–agarose beads preblocked with salmon sperm DNA (Upstate/Millipore, Billerica, MA).

    Control:

    Article Title: Influenza A virus exploits the motility of membrane cytoskeletal actomyosin filaments for its genome packaging in the host cell
    Article Snippet: .. After labelling with the primary antibody, the cells were washed with fresh PBS three times for 5 min each and then incubated with 10 nm gold-conjugated goat anti-rabbit IgG (British BioCell International, UK) for 2 h. As a control for immunolabelling, nonimmune rabbit serum (Invitrogen MA USA) was used instead of primary antibody. ..

    Electrophoretic Mobility Shift Assay:

    Article Title: Dual Roles for Prox1 in the Regulation of the Chicken β B1-Crystallin Promoter
    Article Snippet: .. Oligonucleotides Used for Electrophoretic Mobility Shift Analysis (Sense Strands Only Are Shown) Chromatin Immunoprecipitation Analysis Lenses and livers were removed from 14-day-old chicken embryos and sheared, and formaldehyde cross-linked chromatin (average size, 600 bp) was generated by sonication, as described in Yang et al. 39 Aliquots of chromatin prepared from 10 lenses or 1 to 2 mg liver were incubated with 1 μ L well-characterized rabbit polyclonal antibody prepared against the homeo- and Prospero domains of Prox1 20 or nonimmune rabbit serum (catalog no. 31884; Pierce, Rockford, IL). .. Antibody–chromatin complexes were captured by the addition of 100 μ L protein G–agarose beads preblocked with salmon sperm DNA (Upstate/Millipore, Billerica, MA).

    Chromatin Immunoprecipitation:

    Article Title: Dual Roles for Prox1 in the Regulation of the Chicken β B1-Crystallin Promoter
    Article Snippet: .. Oligonucleotides Used for Electrophoretic Mobility Shift Analysis (Sense Strands Only Are Shown) Chromatin Immunoprecipitation Analysis Lenses and livers were removed from 14-day-old chicken embryos and sheared, and formaldehyde cross-linked chromatin (average size, 600 bp) was generated by sonication, as described in Yang et al. 39 Aliquots of chromatin prepared from 10 lenses or 1 to 2 mg liver were incubated with 1 μ L well-characterized rabbit polyclonal antibody prepared against the homeo- and Prospero domains of Prox1 20 or nonimmune rabbit serum (catalog no. 31884; Pierce, Rockford, IL). .. Antibody–chromatin complexes were captured by the addition of 100 μ L protein G–agarose beads preblocked with salmon sperm DNA (Upstate/Millipore, Billerica, MA).

    Negative Control:

    Article Title: Staphylococcal Enterotoxin B Causes Proliferation of Sensory C-Fibers and Subsequent Enhancement of Neurogenic Inflammation in Rat Skin
    Article Snippet: Goat IgG polyclonal antibody to rabbit IgG (Jackson ImmunoResearch Laboratories), mouse IgG polyclonal antibody to goat IgG (Chemicon International), and goat IgG polyclonal antibody to mouse IgG1 (Invitrogen) conjugated with Cy3 were used as a second step reagent for indirect immunofluorescent staining. .. Nonimmune goat IgG (Chemicon International) or nonimmune rabbit serum (Pierce Biotechnology) was used as a negative control for a primary antibody. ..



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    LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Journal: American Journal of Cancer Research

    Article Title: REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy

    doi:

    Figure Lengend Snippet: LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

    Article Snippet: Antibodies were used in ChIP assays as follows: anti-REST (Millipore, 17-641) and nonimmune rabbit serum IgG (Alpha Diagnostic International).

    Techniques: ChIP-sequencing, Binding Assay, Amplification, Quantitative RT-PCR